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| Picture of the day |
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| Magazine Title : |
Iranian Journal of Toxicology |
| Magazine Vol : |
5 |
| Complete Title of Article : |
Depletion of Ovarian Primordial Follicles after Gamma Irradiation |
| Author : |
Tawakal N, Mubarak Khalid A, Farooq Khan U, Raees Ahmad K, Asmatullsh |
| Educational Part : |
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| Department : |
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| University : |
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| Abstract : |
Introduction: To investigate the total number of primordial follicles, and histological changes in these follicles before and after exposure to gamma radiation.
Material & Method: 108-Female albino rats of 3 weeks age were divided into three groups. Out of them, 36 animals received 4.5 Gray, and 36 received 8.3 Gray of gamma radiation while rest of them received sham radiation. Ovaries of rats were dissected out 2hrs, 4hrs, 6hrs, one day, 7 days and 14 days after irradiation. Histological examination of five random sections of each ovary was performed.
Results: The stockpile of primordial follicles almost depleted within 24 hours. The pool of primordial follicles was greatly suppressed after irradiation showing primordial follicles radiosensitivity. Intense necrosis was evident showing karyorrhexis and karyolysis in nucleus of oocyte while effects on granulosa cells were little and they were added to the bulk of interstitial tissues.
Conclusion: Therefore we can conclude that gamma ionizing radiation induces intense and rapid necrotic degeneration of oocyte in primordial ovarian follicles. The granulosa cells of degenerated primordial follicle increase the bulk of ovarian interstitial tissue. Resultant ovaries do not have enough follicular reserve for ovulatory process which may lead to early cessation of menstruation and eventually infertility.
Key words: Ovary, Oocyte, granulosa cells, necrosis, rat.
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| Introduction : |
To investigate the total number of primordial follicles, and histological changes in these follicles before and after exposure to gamma radiation |
| Methods & Materials : |
108-Female albino rats of 3 weeks age were divided into three groups. Out of them, 36 animals received 4.5 Gray, and 36 received 8.3 Gray of gamma radiation while rest of them received sham radiation. Ovaries of rats were dissected out 2hrs, 4hrs, 6hrs, one day, 7 days and 14 days after irradiation. Histological examination of five random sections of each ovary was performed |
Results : |
The stockpile of primordial follicles almost depleted within 24 hours. The pool of primordial follicles was greatly suppressed after irradiation showing primordial follicles radiosensitivity. Intense necrosis was evident showing karyorrhexis and karyolysis in nucleus of oocyte while effects on granulosa cells were little and they were added to the bulk of interstitial tissues. |
| Conclusion : |
: Therefore we can conclude that gamma ionizing radiation induces intense and rapid necrotic degeneration of oocyte in primordial ovarian follicles. The granulosa cells of degenerated primordial follicle increase the bulk of ovarian interstitial tissue. Resultant ovaries do not have enough follicular reserve for ovulatory process which may lead to early cessation of menstruation and eventually infertility. |
| Date of Site Insertion : |
2010-01-02 |
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